Detection of Talaromyces marneffei from Fresh Tissue of an Inhalational Murine Pulmonary Model Using Nested PCR.
作者： Yinghui Liu , Xiaowen Huang , Xiuwen Yi , Ya He , Eleftherios Mylonakis
DOI: 10.1371/JOURNAL.PONE.0149634
关键词:
摘要: Penicilliosis marneffei, often consecutive to the aspiration of Talaromyces marneffei (Penicillium marneffei), continues be one significant causes morbidity and mortality in immunocompromised patients endemic regions such as Southeast Asia. Improving accuracy diagnosing this disease would aid reducing associated infections. In study, we developed a stable reproducible murine pulmonary model that mimics human penicilliosis using nebulizer deliver (SUMS0152) conidia lungs BALB/c nude mice housed exposure chamber. Using model, further revealed nested PCR was sensitive specific for detecting bronchoalveolar lavage fluid fresh tissues. This inhalation may provide more representative analysis tool studying development addition revealing has predictive value reflecting infection.
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