Controlled surface morphology and hydrophilicity of polycaprolactone toward human retinal pigment epithelium cells

摘要: Abstract Applying scaffolds as a bed to enhance cell proliferation and even differentiation is one of the treatment retina diseases such age-related macular degeneration (AMD) which deteriorating photoreceptors finally happening blindness. In this study, aligned polycaprolactone (PCL) nanofibers were electrospun at different conditions their characteristics measured by scanning electron microscope (SEM) contact angle. Response surface methodology (RSM) was used optimize diameter fabricated nanofibers. Two factors solution concentration voltage value considered independent variables effects on nanofibers' diameters evaluated central composite design optimum obtained 0.12 g/mL 20 kV, respectively. order decrease hydrophobicity PCL, modified alkaline hydrolysis method. Contact time optimized using Box Behnken (120 min 5 M optimal, respectively). angle measurement showed high hydrophilicity treated (with 7.48°). Plasma applied compare effect two kinds modification methods simultaneously hydrolyzed scaffolds. The RPE cells grown examined immunocytochemistry (ICC), MTT continuous inspection cellular morphology. Interestingly, Human revealed characteristic morphology scaffold well. As result, we introduced culture substrate with low (185.8 nm), porosity (82%) suitable 7.48 degree) can be promising for hRPE transplantation.