Enhancement of chondrocyte proliferation, distribution, and functions within polycaprolactone scaffolds by surface treatments
作者： Paweena Uppanan , Boonlom Thavornyutikarn , Wasana Kosorn , Pakkanun Kaewkong , Wanida Janvikul
DOI: 10.1002/JBM.A.35370
关键词: Scaffold 、 Scanning electron microscope 、 Biophysics 、 Hydrolysis 、 Glycosaminoglycan 、 Biomedical engineering 、 Type II collagen 、 Polycaprolactone 、 Contact angle 、 Chondrocyte 、 Materials science
摘要: Enhancement of porcine chondrocyte growth, distribution and functions within polycaprolactone (PCL) scaffolds was attempted using alkaline hydrolysis oxygen plasma treatment. The PCL performed either before or after scaffold fabrication in the preparations pre-hydrolyzed (pre-HPCL) post-HPCL scaffolds, respectively. PCL, pre-HPCL, were subsequently plasma-treated to yield All comparatively characterized, terms surface morphology, hydrophilicity, atomic composition scanning electron microscopy, contact angle measurement X-ray photoelectron spectroscopy, interactions chondrocytes with individual assessed, cartilage-gene expression cartilaginous matrix production reverse transcription polymerase chain reaction analysis glycosaminoglycans (GAGs) assay, cell infiltration investigated by histological immunofluorescence analysis. results revealed that treatment exhibited a more prominent effect on enhancement roughness hydrophilicity than hydrolysis. subjected both treatments stimulated cells secret GAGs type II collagen. sequence also evidently played crucial role materials cultured chondrocytes. prior fabrication, followed resulting fabricated scaffold, yielded pre-HPCL homogeneous hydrophilic characteristics all over material. Consequently, could proliferate well, infiltrate most deeply ultimately produce highest amounts cartilage-specific substances throughout this scaffold.
sci-hub.se 参考文章(37)
David Eyre, Articular cartilage and changes in Arthritis: Collagen of articular cartilage Arthritis Research & Therapy. ,vol. 4, pp. 30- 35 ,(2001) , 10.1186/AR380
M. Lebourg, J. Suay Antón, J.L. Gómez Ribelles, Porous membranes of PLLA–PCL blend for tissue engineering applications European Polymer Journal. ,vol. 44, pp. 2207- 2218 ,(2008) , 10.1016/J.EURPOLYMJ.2008.04.033
Robin A. Quirk, Richard M. France, Kevin M. Shakesheff, Steven M. Howdle, Supercritical fluid technologies and tissue engineering scaffolds Current Opinion in Solid State & Materials Science. ,vol. 8, pp. 313- 321 ,(2004) , 10.1016/J.COSSMS.2003.12.004
GA Hutcheon, C Messiou, RM Wyre, MC Davies, S Downes, Water absorption and surface properties of novel poly(ethylmethacrylate) polymer systems for use in bone and cartilage repair. Biomaterials. ,vol. 22, pp. 667- 676 ,(2001) , 10.1016/S0142-9612(00)00229-5
Y WAN, C TU, J YANG, J BEI, S WANG, Influences of ammonia plasma treatment on modifying depth and degradation of poly(L-lactide) scaffolds. Biomaterials. ,vol. 27, pp. 2699- 2704 ,(2006) , 10.1016/J.BIOMATERIALS.2005.12.007
Maria Ann Woodruff, Dietmar Werner Hutmacher, The return of a forgotten polymer—Polycaprolactone in the 21st century Progress in Polymer Science. ,vol. 35, pp. 1217- 1256 ,(2010) , 10.1016/J.PROGPOLYMSCI.2010.04.002
A. Getgood, T.P.S. Bhullar, N. Rushton, Current concepts in articular cartilage repair Orthopaedics and Trauma. ,vol. 23, pp. 189- 200 ,(2009) , 10.1016/J.MPORTH.2009.05.002
Johnna S Temenoff, Antonios G Mikos, Review: tissue engineering for regeneration of articular cartilage Biomaterials. ,vol. 21, pp. 431- 440 ,(2000) , 10.1016/S0142-9612(99)00213-6
Barbara D Boyan, Thomas W Hummert, David D Dean, Zvi Schwartz, Role of material surfaces in regulating bone and cartilage cell response Biomaterials. ,vol. 17, pp. 137- 146 ,(1996) , 10.1016/0142-9612(96)85758-9
Tinneke Jacobs, Nathalie De Geyter, Rino Morent, Tim Desmet, Peter Dubruel, Christophe Leys, Plasma treatment of polycaprolactone at medium pressure Surface & Coatings Technology. ,vol. 205, ,(2011) , 10.1016/J.SURFCOAT.2011.02.012