Chondrogenic phenotype in responses to poly(ɛ-caprolactone) scaffolds catalyzed by bioenzymes: effects of surface topography and chemistry

摘要: Biodegradable poly(e-caprolactone) (PCL) has been increasingly investigated as a promising scaffolding material for articular cartilage tissue repair. However, its use can be limited due to surface hydrophobicity and topography. In this study, 3D porous PCL scaffolds fabricated by fused deposition modeling (FDM) machine were enzymatically hydrolyzed using two different biocatalysts, namely Novozyme®435 Amano lipase PS, at varied treatment conditions in pH 8.0 phosphate buffer solution. The improved topography chemistry of the anticipated ultimately boost growth porcine chondrocytes promote chondrogenic phenotype during cell culture. Alterations roughness, wettability, after enzymatic thoroughly several techniques, e.g., SEM, AFM, contact angle energy measurement, XPS. With increasing enzyme content, incubation time, temperature, surfaces became rougher more hydrophilic. addition, was found have higher activity than PS when both used same condition. Interestingly, degradation process rarely induced deterioration compressive strength bulk slightly reduced molecular weight filament surface. After 28 days culture, catalyzed could facilitate not only proliferate properly, but also function effectively, compared with non-modified scaffold. Furthermore, treatments 50 mg 25 °C from 10 min 60 min evidently proven provide optimally enhanced roughness hydrophilicity most significantly favorable induction phenotype, indicated greatest expression level cartilage-specific gene largest production total glycosaminoglycans.