Protective effects of calcium channel blockers against free radical-impaired endothelial cell proliferation
作者： I.Tong Mak , Patricia Boehme , William B. Weglicki
DOI: 10.1016/0006-2952(95)02039-X
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摘要: Abstract We have shown previously that the lipophilic calcium channel blockers exhibit membrane antioxidant activity. In present study, when attached bovine aortic endothelial cells were exposed for 20 min to a low concentration of oxy-radicals generated from dihydroxyfumarate + Fe-ADP, no loss glutathione or viability was detected; however, cell number, determined 48 hr later by tetrazolium salt MTT assay, decreased 45% controls. Treatment 1 with (2–20 μM) prior free radical exposure protected against impaired growth in concentration-dependent manner. The order potency ricardipine ⩾ nifedipine verapamil > diltiazem, which appears parallel their potency. addition, (+)-nicardipine, and its pharmacologically inactive isomer, (−)-nicardipine, similarly effective. conclude it activity preserved capacity free-radical damage; such protective effects may contribute antiatherogenic effects.
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