An enhanced hTERT promoter-driven CRISPR/Cas9 system selectively inhibits the progression of bladder cancer cells.
作者： Xinbo Huang , Chengle Zhuang , Changshui Zhuang , Tiefu Xiong , Yawen Li
DOI: 10.1039/C7MB00354D
关键词:
摘要: The current therapies for treating tumors are lacking in efficacy and specificity. Synthetic biology principles may bring some new possible methods curing cancer. Here we present a synthetic logic circuit based on the CRISPR/Cas9 system. technology has been applied many biological fields, including cancer research. In this study, expression of Cas9 nuclease was controlled indirectly by an enhanced hTERT promoter using GAL4/upstream activating sequence (UAS) binding driven 5XUAS, single guide RNA (sgRNA) used to target mutant or wild-type HRAS, fusion gene GAL4-P65 promoter. system tested bladder cells (T24 5637) results showed that could drive these cell lines. Then all devices were packed into lentivirus quantitative real-time PCR mRNA level HRAS selectively inhibited T24 5637 cells. functional experiments suggested proliferation, migration invasion suppressed, apoptosis rate increased but not human foreskin fibroblasts (HFF). conclusion, successfully constructed promoter-driven data it suppress progression
rsc.org
sci-hub.se 参考文章(38)
Deborah Saraggi, Roberto Salmaso, Elisa Valentini, Giada Munari, Vincenzo Vindigni, Massimo Rugge, Matteo Fassan, Lorenzo Cerroni, None, Pigmented trichoblastoma developed in a sebaceous nevus: HRAS mutation as a common molecular driver. Pathology Research and Practice. ,vol. 213, pp. 860- 862 ,(2017) , 10.1016/J.PRP.2017.03.012
Elena Eremeeva, Michail Abramov, Lia Margamuljana, Piet Herdewijn, Base-Modified Nucleic Acids as a Powerful Tool for Synthetic Biology and Biotechnology. Chemistry: A European Journal. ,vol. 23, pp. 9560- 9576 ,(2017) , 10.1002/CHEM.201700679
A. Biagioni, A. Chillà, E. Andreucci, A. Laurenzana, F. Margheri, S. Peppicelli, M. Del Rosso, G. Fibbi, Type II CRISPR/Cas9 approach in the oncological therapy. Journal of Experimental & Clinical Cancer Research. ,vol. 36, pp. 80- 80 ,(2017) , 10.1186/S13046-017-0550-0
Shao-Chen Pang, Hou-Peng Wang, Zuo-Yan Zhu, Yong-Hua Sun, Transcriptional Activity and DNA Methylation Dynamics of the Gal4/UAS System in Zebrafish Marine Biotechnology. ,vol. 17, pp. 593- 603 ,(2015) , 10.1007/S10126-015-9641-0
B.H. Liu, Y. Yang, J.F.R. Paton, F. Li, J. Boulaire, S. Kasparov, S. Wang, GAL4–NF-κB Fusion Protein Augments Transgene Expression from Neuronal Promoters in the Rat Brain Molecular Therapy. ,vol. 14, pp. 872- 882 ,(2006) , 10.1016/J.YMTHE.2006.05.020
Sumit Borah, Linghe Xi, Arthur J. Zaug, Natasha M. Powell, Garrett M. Dancik, Scott B. Cohen, James C. Costello, Dan Theodorescu, Thomas R. Cech, TERT promoter mutations and telomerase reactivation in urothelial cancer Science. ,vol. 347, pp. 1006- 1010 ,(2015) , 10.1126/SCIENCE.1260200
Kian-Huat Lim, Antonio T. Baines, James J. Fiordalisi, Michail Shipitsin, Larry A. Feig, Adrienne D. Cox, Channing J. Der, Christopher M. Counter, Activation of RalA is critical for Ras-induced tumorigenesis of human cells Cancer Cell. ,vol. 7, pp. 533- 545 ,(2005) , 10.1016/J.CCR.2005.04.030
Liang Cheng, Darrell D. Davison, Julia Adams, Antonio Lopez-Beltran, Lisha Wang, Rodolfo Montironi, Shaobo Zhang, Biomarkers in bladder cancer: Translational and clinical implications Critical Reviews in Oncology Hematology. ,vol. 89, pp. 73- 111 ,(2014) , 10.1016/J.CRITREVONC.2013.08.008
Yuchen Liu, Yayue Zeng, Li Liu, Chengle Zhuang, Xing Fu, Weiren Huang, Zhiming Cai, Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells Nature Communications. ,vol. 5, pp. 5393- ,(2014) , 10.1038/NCOMMS6393
Song Wu, Peide Huang, Chong Li, Yi Huang, Xianxin Li, Yongqiang Wang, Chao Chen, Zhaojie Lv, Aifa Tang, Xiaojuan Sun, Jingxiao Lu, Weiping Li, Jie Zhou, Yaoting Gui, Fangjian Zhou, Daping Wang, Zhiming Cai, Telomerase Reverse Transcriptase Gene Promoter Mutations Help Discern the Origin of Urogenital Tumors: A Genomic and Molecular Study European Urology. ,vol. 65, pp. 274- 277 ,(2014) , 10.1016/J.EURURO.2013.10.038