DNA sequence analysis of the transposon Tn3: three genes and three sites involved in transposition of Tn3.

摘要: Abstract The complete nucleotide sequence of the transposon Tn3 and 20 mutations which affect its transposition are reported. mutations, generated in vitro by random insertion synthetic restriction sites, proved to contain small duplications or deletions immediately adjacent new site. By determining phenotype DNA these we were able generate an overlapping phenotypic map. This 4957 bp encodes three polypeptides account for all but 350 total coding capacity. These proteins transposase, a high molecular weight polypeptide (1015 amino acids) encoded tnpA gene; Tn3-specific repressor, low (185 tnpR 286 acid β-lactamase. 38 inverted repeats flanking appear be absolutely required cis transpose. Genetic data suggest that contains third site (Gill et al., 1978), designated IRS (internal resolution site), whose absence results two copies as direct into recipient DNA. We intermediates transposition, is recombination subsequent segregation leave single copy 1978). A 23 within terminus transposase shares strong homology with repeat may internal