Early events in the replication of Mu prophage DNA.

摘要: To determine whether the early replication of Mu prophage DNA proceeds beyond termini into hose DNA, amounts both and prophage-adjacent host sequences were measured using a DNA-DNA annealing assay after induction vegetative cycle. Whereas Mu-specific synthesis began 6 to 8 min induction, no amplification adjacent was observed. These data suggest that Mu-induced is constrained within boundaries prophage. Since does not undergo lambda-like excision from its original site (E. Ljungquist A. I. Bukhari, Proc. Natl. Acad. Sci. U.S.A. 74:3143--3147, 1977), we propose existence control mechanism which excludes initial mu replication. The frequencies sequences, relative other Escherichia coli genes, observed change onset This suggests these regions remain associated with chromosome continue be replicated by chromosomal fork. Therefore, conclude are maintained in chromosome, rather than on an extrachromosomal form containing DNA.