A single-cell translocation and secretion assay (TransSeA).

摘要: Understanding biological heterogeneity at the single cell level is required for advancing insights into complexity of human physiology and diseases. While advances in technological analytical methods have afforded unprecedented glimpses this heterogeneity, information captured to date largely represents one-time “snap” shots physiology. To address limits existing accelerate discoveries from studies, we developed a single-cell translocation secretion assay (TransSeA) that supports time lapse analysis, enables molecular cargo analysis secretions such as extracellular vesicles (EVs) cells, allows massively parallel transfer according user-defined selection criteria, tracking phenotypes between parental progeny cells derived cells. demonstrate unique capabilities efficiencies assay, present studies related secretions, EV cargos intrinsic properties. Although used examples feasibility versatility technology, already provided key unanswered questions microRNAs carried by EVs, relationships rates gene expressions, spontaneous, trans-generational phenotypic changes