Functional Investigation of NCI-H460-Inducible Myofibroblasts on the Chemoresistance to VP-16 with a Microfluidic 3D Co-Culture Device

摘要: Fibroblasts, the major cell type in tumor stroma, are essential for growth and survival, represent an important therapeutic target cancers. Here we presented a microfluidic co-culture device which three-dimensional (3D) matrix was employed to reconstruct vivo-like fibroblast-tumor microenvironment investigation of role myofibroblasts induced by lung cancer cells chemoresistance VP-16. Composed double-layer chip injection pump, houses fibroblasts co-cultured 3D 2D mode induce become with supplement medium continuously. With this device, verified that cytokines secreted could effectively transform into myofibroblasts. Moreover, compared fibroblasts, showed higher resistance anticancer drug We also demonstrated kind acquired associated expression Glucose-regulated protein 78 (GP78). concluded allows assay characterize various cellular events single sequentially, facilitating better understanding interactions among heterotypic sophisticated microenvironment.