Urinary Peptidomic Analysis Identifies Potential Biomarkers for Acute Rejection of Renal Transplantation

摘要: Human urine is a complex matrix of proteins, endogenous peptides, lipids, and metabolites. The level any or all these components can reflect the pathophysiological status an individual especially kidney at time collection. naturally occurring urinary peptides which are thought to be product several proteolytic degradation processes may provide clinically useful biomarkers for different renal systemic diseases. To examine if specific differences in peptidome (<10 kDa) occur acute transplant rejection (AR), we undertook study samples collected from biopsy-proven AR (n = 10), stable graft function healthy normal control 10). were extracted fractionated with high-performance liquid chromatography followed by matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometric (MS) analysis. We identified 54 including multiple Tamm–Horsfall protein (UMOD). A panel discriminate patients graft. have shown that MALDI tool identify potential biomarkers, after verification larger patient cohort used as non-invasive monitoring rejection.