Different molecular mechanisms are involved in the multihormonal control of glucose transport in FRTL5 rat thyroid cells

摘要: We investigated the molecular mechanisms by which TSH, insulin and IGF-1 modulate glucose transport system in FRTL5 cells. found that increased transporter Glut-1 specific mRNA levels 6, 8 5 fold over control, respectively. The effect on was evident after 2 hours, followed an protein expression whole cells, as judged western blot analysis, hours of stimulation with all hormones studied. In contrast, plasma membrane (300–400% control) TSH (10 mU/ml), dibutyrryl-cAMP (1mM), ng/ml) nM). These data indicate is under multihormonal control Two different are involved transport: a) neosynthesis activation gene expression; b) recruitment carriers from intracellular pool to membrane.