Evidence that translocation of the glucose transport activity is the major mechanism of insulin action on glucose transport in fat cells.

摘要: The glucose transport activity associated with the plasma membrane-rich and Golgi-rich fractions of fat cells was determined after they were reconstituted into egg lecithin liposomes. When two subcellular isolated under conditions that would minimize their cross-contamination, in fraction found to be increased 6.3- 8.6-fold by insulin, which added before homogenization, reduced approximately one-half. In this study, (either basal or plus insulin state) solubilized, reconstituted, assayed an overall efficiency 25-35%. Four agents known have insulin-like effects on intact (hydrogen peroxide, sodium vanadate, trypsin, p-chloromercuriphenyl sulfonate) not only fraction, but also decreased fraction. effect hydrogen unlike abolished when receptor modified proteolytically. Upon administration cells, subsequent elimination hormone, activities affected almost concomitantly towards opposite directions. It is proposed as a working hypothesis translocation system membrane from major, if sole, mechanism stimulates cells.