Interactions between structural and chemical biomimetism in synthetic stem cell niches

摘要: Advancements in understanding stem cell functions and differentiation are of key importance for the clinical success stem-cell-based therapies. 3D structural niches fabricated by two-photon polymerization a powerful platform controlling growth differentiation. In this paper, we investigate possibility further fate tuning mechanical properties such through coating with thin layers biomimetic hyaluronan-based gelatin-based hydrogels. We first assess biocompatibility chemical coatings then study interactions between biomimetism on response MSCs terms proliferation observed clear effect hydrogel otherwise identical scaffolds. particular, gelatin-coated stronger metabolic activity commitment toward osteo-chondral lineage respect to hyaluronan-coated niches. Conversely, reduction homing was all coated niches, especially This demonstrates feasibility independently different cues, bioengineered i.e. scaffold geometry surface stiffness. will allow, one hand, their specific role and, other finely synergistic effect.