Concentration of nucleotides and deoxynucleotides in peripheral and phytohemagglutinin-stimulated mammalian lymphocytes: Effects of adenosine and deoxyadenosine
作者： G.J. Peters , R.A. de Abreu , A. Oosterhof , J.H. Veerkamp
DOI: 10.1016/0304-4165(83)90182-4
关键词:
摘要: Concentrations of purine and pyrimidine ribonucleotides were measured with HPLC in lymphocytes man, horse, pig sheep rat thymocytes. The ATP concentration was highest all species about 850 pmol/10(6) cells human equine lymphocytes, higher porcine lower ovine GTP comparable human, but lymphocytes. also horse a luciferin-luciferase assay. During culturing or without phytohemagglutinin the concentrations decreased these TTP dATP DNA polymerase Phytohemagglutinin-stimulation increased three species, only ATP, thymidine incorporation phytohemagglutinin-stimulated after 24 48 h presence adenosine deoxyadenosine. Adenosine equine, not Deoxyadenosine did affect concentration. EHNA it other conditions 3-, 10-, 9-fold, respectively, considerably incorporation. 2-5-fold, man stimulated 5-fold. latter results indicate that accumulation is always associated inhibition cell proliferation.
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