Is cholesterol and amyloid-β stress induced CD147 expression a protective response? evidence that extracellular cyclophilin a mediated neuroprotection is reliant on CD147

摘要: The CD147 protein is a ubiquitous multifunctional membrane receptor. Expression of CD147, which regulated by sterol carrier protein, reportedly modulates amyloid-β (Aβ), the neurotoxic peptide implicated in neuronal degeneration Alzheimer's disease (AD). Given that high fat/cholesterol linked to amyloid deposition AD, we investigated if cholesterol and/or Aβ can alter expression rat cortical cultures. Water-soluble and Aβ42 dose-dependently increased expression, but reduced FL-AβPP expression. Cholesterol treatment also lactate dehydrogenase release varying degrees. Upregulation was probably mediated oxidative stress, as H2O2 (3 μM) induced In light these findings, induction cytoprotective, compensatory response injury, or alternatively, cell death signal. To this end, used recombinant adenovirus overexpress human (in SH-SY5Y cells primary neurons), pre-treated cultures with without cyclophilin A (rCYPA) prior exposure. We showed protected against Aβ42, only when rCYPA added Together, our findings reveal potentially important relationships between loading, toxicity, putative involvement CYPA neuroprotection AD.