Integrative assessment of potential effects of dioxins and related compounds in wild Baikal seals (Pusa sibirica): application of microarray and biochemical analyses.

摘要: We have previously indicated that accumulation of chlorinated dioxins and related compounds (DRCs) induced cytochrome P450 (CYP) 1A1, 1A2 1B1 isozymes in the liver wild Baikal seals (Pusa sibirica). Here we attempt to assess potential effects DRCs triggered by induction these CYP1 this species, using an integrative approach, combining gene expression monitoring biochemical assays. To screen genes may potentially respond exposure DRCs, constructed a custom cDNA oligo array can target mRNAs seals, monitored hepatic mRNA levels population. Correlation analyses between total 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalents (TEQs) supported our previous findings high induces transcription CYP1A1, CYP1A2 CYP1B1 genes. In addition, assessment chronic alter transcript oxidative stress, Fe ion homeostasis, inflammatory responses. The showed significant positive correlations with malondialdehyde, biomarker lipid peroxidation, etheno-dA, DNA adduct, suggesting peroxidation be enhanced through production reactive oxygen species (ROS) induction. Moreover, there was correlation heme oxygenase activities malondialdehyde levels, prompted degradation ROS. Fetuin-A which are suppressed inflammation, negative TEQ hepcidin conversely increased had etheno-dA implying progression inflammation DRC-induced stress. Taken together, propose here suffer from on isozymes, followed inflammation.