Increased potential for NAD(P)H-dependent reactive oxygen species production of hepatic subcellular fractions of fish species with in vivo exposure to contaminants.
作者： D.R Livingstone , C.L Mitchelmore , S.C.M O'Hara , P Lemaire , J Sturve
DOI: 10.1016/S0141-1136(00)00043-X
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摘要: The present study investigated the proposed involvement of contaminant-stimulated reactive oxygen species (ROS) production in disease processes fish. NAD(P)H-dependent ROS subcellular fractions was determined by iron/EDTA-mediated oxidation 2-keto-4-methiolbutyric acid. Hepatic cytosolic NADPH-dependent and microsomal were increased 51--160% (P < 0.05) rainbow trout (Oncorhynchus mykiss) 15 weeks after a single i.p. injection polychlorobiphenyl (PCB) (100 mg Clophen A50 kg -1 wet wt.). NADH-dependent 114% higher perch (Perca fluviatilis) from PCB-contaminated Lake Jarnsjon compared to clean Vanern, Sweden. mitochondrial NADH-dependent, variously elevated up 160% flounder (Platichthys flesus) at various sites along two pollution transects near ports Rotterdam Amsterdam, Netherlands. Overall data indicate potential for liver fish exposed field pollution, support hypothesis oxidative stress as mechanism contaminant-mediated
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