Extracellular matrix proteins modulate asthmatic airway smooth muscle cell proliferation via an autocrine mechanism.

摘要: BACKGROUND: Airway remodeling is a key feature of persistent asthma and includes alterations in the extracellular matrix protein profile around airway smooth muscle (ASM) hyperplasia ASM. We have previously shown that nonasthmatic ASM cells culture produce range proteins asthmatic proliferate faster than from patients. OBJECTIVE: In this study, we compared produced by cells. also examined influence these conditioned medium derived or on proliferation METHODS: Extracellular were measured ELISA; was tritiated thymidine incorporation. RESULTS: Production perlecan collagen I patients significantly increased. contrast, laminin alpha1 IV decreased. Chondroitin sulfate detectable only Compared with proteins, enhanced cell proliferation. Conditioned did not induce greater CONCLUSIONS: The data show components altered soluble mediators secreted has potential to These changes are likely contribute wall occurs asthma.