Mechanism of mononuclear cell activation by an anti-CD43 (sialophorin) agonistic antibody.

摘要: CD43 (sialophorin, gpL115) is a sialoglycoprotein expressed on wide variety of blood cells including lymphocytes, monocytes, neutrophils, and platelets. L10, an anti-CD43 mAb, has been shown to induce monocyte-dependent activation proliferation human T lymphocytes. We have studied the signaling mechanism involved in this process. Treatment PBMC purified populations monocytes with L10 induced hydrolysis phosphoinositides resultant generation phosphoinositide-derived second messengers diacylglycerol inositol phosphates. This was associated translocation protein kinase C from cytosol membrane fractions increase free intracellular Ca2+ treated cells. In leukemic cell lines, magnitude via did not correlate density TCR/CD3 surface expression nor intensity TCR/CD3. Moreover, mutant derived line HPB-ALL that severely defective nevertheless had normal compared parent line. It concluded functionally coupled phospholipase C/phosphoinositides pathway. cells, proceeds independently The widespread suggests potentially important role for molecule orchestrating multiple types.