Induced resistance in the human non small cell lung carcinoma (NCI-H460) cell line in vitro by anticancer drugs.
作者： M. Pesic , J.Z. Markovic , D. Jankovic , S. Kanazir , I.D. Markovic
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摘要: Exposure of human non-small cell lung cancer cells (NCI-H460) to gradually increasing concentrations doxorubicin resulted in the appearance a new line (NCI-H460/R) that was resistant (96.2-fold) and cross-resistant etoposide, paclitaxel, vinblastine epirubicin. Slight cross-resistance two MDR-unrelated drugs 8-Cl-cAMP sulfinosine observed. Flow cytometry analysis showed accumulation 88.4% lower than parental cells. Also, verapamil significantly decreased efflux rate NCI-H460 NCI-H460/R cells, whereas curcumin inhibited only. Gene expression data confirmed induction mdr1 (P-gp), as judged by observed 15-fold increase its mRNA concentration doxorubicin-resistant In contrast, mrp1 lrp unaffected resistance. Further work should develop rationale for novel treatment NSCLC with appropriate modulators resistance aimed at improving outcome acquired drug
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