Uracil influences quorum sensing and biofilm formation in Pseudomonas aeruginosa and fluorouracil is an antagonist
作者： Akihiro Ueda , Can Attila , Marvin Whiteley , Thomas K. Wood
DOI: 10.1111/J.1751-7915.2008.00060.X
关键词:
摘要: Pseudomonas aeruginosa is an ubiquitous, opportunistic pathogen whose biofilms are notoriously difficult to control. Here we discover uracil influences all three known quorum‐sensing (QS) pathways of P. aeruginosa. By screening 5850 transposon mutants for altered biofilm formation, identified seven uracil‐related mutations that abolished formation. Whole‐transcriptome studies showed the (e.g. pyrF catalyses last step in uridine monophosphate synthesis) alter regulation QS [LasR‐, RhlR‐ and 2‐heptyl‐3‐hydroxy‐4‐quinolone (PQS)‐related regulons]; addition extracellular restored global wild‐type regulation. Phenotypic confirmed LasR (elastase), RhlR (pyocyanin, rhamnolipids), PQS swarming regulons. Our results also demonstrate virulence (the mutant was less virulent barley). Additionally, found anticancer analogue, 5‐fluorouracil, repressed phenotypes reduced virulence. Hence, have a central regulator important potential novel class efficacious drugs controlling cellular behaviour formation virulence).
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