Differential utilization of Janus kinase-signal transducer activator of transcription signaling pathways in the stimulation of human natural killer cells by IL-2, IL-12, and IFN-alpha.

摘要: IL-2-, IL-12-, and IFN-alpha-mediated signaling pathways were analyzed in primary NK cells the NK3.3 cell line. Gel mobility shift immunoprecipitation analyses revealed that addition to activating STAT3 (signal transducer activator of transcription-3) STAT5, IL-2 induced tyrosine serine phosphorylation STAT1 alpha, which formed IFN-gamma-activated sequence-binding complexes by itself with STAT3. Although IFN-alpha activated alpha predominantly while alpha. less activation greater STAT5 compared preactivated cells. In cells, comparable formation c-fos promoter sis-inducible element containing alone it preferentially Thus, is not always identical contrast IFN-alpha, IL-12 strong STAT4 variable weak However, supershift using probe showed STAT4, STAT3, only, or only correlated increased serine, but tyrosine. Finally, JAK1 JAK3, JAK2 TYK2 both Differential consequent differential separate overlapping STAT proteins IL-2, IL-12, may provide a molecular basis for similarities differences actions these cytokines on