Investigation of the physiological response to oxygen limited process conditions of Pichia pastoris Mut+ strain using a two-compartment scale-down system

摘要: Inhomogeneities in production-scale bioreactors influence microbial growth and product quality due to insufficient mixing mass transfer. For this reason, lots of efforts are being made investigate the effects gradients that impose stress large-scale reactors laboratory scale. We have implemented a scale-down model which allows separating homogeneous part, stirred tank reactor (STR), plug flow (PFR) mimics inhomogeneous regimes fermenters. This shows solutions trigger oxygen limited conditions PFR part setup for physiological analysis. The goal study was scale-up relevant responses Pichia pastoris strain process above mentioned two-compartment bioreactor setup. Experimental results with non-induced cultures show specific rate significantly decreased increasing exposure time limitation. In parallel more by-products were produced. Examining scalable key parameters, multivariate data analyses solely using on-line revealed different exposures limitation affected culture performance. work small scale-downs reflects new approaches valuable development tool accelerating characterization or verifying CFD simulations bioreactors. As novel methodological achievement, combination system proposed techniques is recognition on performance bioprocess issues.