Roles of Epithelial Cell–Derived Type 2–Initiating Cytokines in Experimental Allergic Conjunctivitis

摘要: PURPOSE To clarify the possible involvement of type 2-initiating cytokines interleukin (IL)-25, IL-33, and thymic stromal lymphopoietin (TSLP) in pathophysiology allergic conjunctivitis, we evaluated ragweed (RW)-induced experimental conjunctivitis (EAC) models by using IL-25 knockout (KO), IL-33 KO, TSLP receptor (TSLPR) KO mice. METHODS Interleukin-25 TSLPR BALB/c wild-type mice were sensitized twice with RW alum then challenged eye drops. Clinical scores eosinophil infiltration evaluated. Expression levels serum immunoglobulin E (IgE) conjunctival tissues quantified immunohistochemical analysis was carried out. RESULTS Significant reductions clinical numbers infiltrating eosinophils observed RW-EAC model There no significant differences among IL-25KO, Serum IgE concentration upregulated after challenges, there mouse genotypes. il4, il5, il13, ccl5 mRNA diminished conjunctivae compared to those Interleukin-33 expression as early 1 hour eye-drop challenge. The number basophils that CONCLUSIONS Among cytokines, may play a major role inflammation an model.