IL‐33 promotes the induction of immunoglobulin production after inhalation of house dust mite extract in mice

摘要: Background The initial immune response to house dust mite (HDM) is orchestrated by an interplay between epithelial cells (ECs) and dendritic (DCs). Innate cytokines released HDM-exposed ECs activate airway DCs effector inflammatory cells, which together induce a HDM-specific Th2 cell response. Here, we investigate the respective roles of IL-33 in sensitization HDM. Method Balb/c mice were exposed via airways different HDM extracts, differing at least endotoxin levels [Lotox (LT) HiTox (HT)]. Alternatively, HDM-pulsed presence or absence additional LT-HDM, administration LT-HDM plus recombinant IL-33, intratracheally (i.t.) administered allergic inflammation. Eosinophil recruitment, cytokine production, serum immunoglobulins, histology analyzed. Results Direct exposure with HT-HDM induced eosinophilic inflammation, increase total IgE IgG1, while was not able do so. In contrast, i.t. instillation LT-HDM-pulsed similar mucus but IgG1 induced. Administration supply B unprocessed antigen, sufficient antibody production. Simultaneous response, besides cellular response. Conclusion These results demonstrate that drive needed humoral single inhalational challenge HDM.