Multivitamins, Folate, and Green Vegetables Protect against Gene Promoter Methylation in the Aerodigestive Tract of Smokers

摘要: One promising approach for early detection of lung cancer is by monitoring gene promoter hypermethylation events in sputum. Epidemiologic studies suggest that dietary fruits and vegetables the micronutrients they contain may reduce risk cancer. In this study, we evaluated whether diet multivitamin use influenced prevalence methylation cells exfoliated from aerodigestive tract current former smokers. Members (N = 1,101) Lovelace Smokers Cohort completed Harvard Food Frequency Questionnaire provided a sputum sample was assessed eight genes commonly silenced associated with disease. Methylation status categorized as low (fewer than two methylated) or high (two more methylated). Logistic regression models were used to identify associations between 21 variables hypothesized affect acquisition methylation. Significant protection against observed leafy green [odds ratio (OR) 0.83 per 12 monthly servings; 95% confidence interval (95% CI), 0.74-0.93] folate (OR, 0.84 750 microg/d; CI, 0.72-0.99). Protection also seen multivitamins 0.57; 0.40-0.83). This first cohort-based study factors reduced airway epithelium Novel interventions prevent should be developed based on ability supplements reprogramming epigenome.