Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

摘要: Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in prevalence diabetes, soaring cost managing diabetes its complications underscores an urgent need for safer, more efficient affordable alternative treatments. Over 1200 plant species reported ethnomedicine treating these represents important promising source identification novel antidiabetic compounds. Evaluating medicinal plants desirable bioactivity goes hand-in-hand with methods analytical biochemistry separating identifying lead This review aims to provide comprehensive summary current used research form useful resource researchers beginning field. The summarises understanding blood glucose regulation general mechanisms action medications, combines knowledge on common experimental approaches screening extracts activity currently available technologies separation bioactive natural products. Common vivo animal models, vitro silico biochemical assays testing effects discussed particular emphasis such cell-based bioassays insulin secretagogues insulinomimetics. Enzyme inhibition molecular docking also highlighted. role metabolomics, metabolite profiling, dereplication data high-throughput discovery agents is reviewed. Finally, this sample preparation techniques liquid–liquid extraction, solid phase supercritical fluid critical function nuclear magnetic resonance high resolution liquid chromatography–mass spectrometry dereplication, putative structure elucidation compounds from evidence-based plants.