Nucleotide sequence of a cluster of genes involved in the transformation of Haemophilus influenzae Rd.

作者: Jean-François Tomb , Hiyam El-Hajj , Hamilton O. Smith

DOI: 10.1016/0378-1119(91)90457-M

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摘要: Abstract A genetic locus implicated in the development of competence Haemophilus influenzae Rd has been previously mapped to a 12.8-kb Pst I region chromosome [Tomb et al. J. Bacteriol. 171 (1989) 3796–3802]. To define boundaries this and identify gene(s) involved transformation, additional mini-Tn 10kan mutagenesis was performed containing all mutagenic insertions sequenced. Three new transformation-deficient (Tfo-) mutants were found, bringing number distinct mutations up eight. The transformation frequency strains carrying 25- 10 5 -fold less than wild type. ends mim-Tn element used as starting points sequence 9.1-kb region. position eight determined ten putative open reading frames (ORFs) found. One elements had inserted an intergenic while rest six ORFs. Based on phenotypes mutant insertions, we concluded that at least three genes should be transformation. In addition, fourteen 9-11-bp uptake signal sequences ( USS ) four which part stem-loop structures could function attenuators or terminators transcription.

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