Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation.

摘要: Cell envelopes of Haemophilus influenzae have been prepared by breakage in a French pressure cell followed differential centrifugation. The envelope fraction may be resolved into an inner-membrane (light) and outer-membrane (heavy) on density gradients. Envelopes from competent cells possess elevated levels lipopolysaccharide with composition different that log-phase envelopes. Three apparently new polypeptides observed gel electrophoresis sodium dodecyl sulfate; additional quantitative alterations the profiles membrane also company development capacity to transport deoxyribonucleic acid. Most polypeptide changes are confined outer membrane; one is associated inner cytoplasmic cells. Protein synthesis during competence developement rquired for change lipopolysaccharides occur.