A high-performance liquid chromatographic method for the determination of nicardipine in plasma and its application to pharmacokinetics in humans.

摘要: A simple and sensitive reversed-phase liquid chromatographic method has been developed validated for the analysis of nicardipine in human plasma study pharmacokinetics drug body. Nicardipine nimodipine (internal standard) were extracted with hexane-butanol (12:1, v/v) after addition borate buffer (0.5 mol/mL, pH = 9.0), then measured by HPLC using a Hypersil C18 column as stationary phase acetonitrile--KH2PO4 (0.015 M, 5.5)--triethylamine mobile phase. was quantified ultraviolet absorbance at 236 nm. The proved to be linear clinical range 5-200 ng/mL regression coefficient 0.9998. lower limit detection 2.5 ng/mL. Intra- inter-day coefficients variation assay 3.5-5.4% (n 7) 5.2-6.4% 5), respectively. recovery 92.8-100.8% plasma. used determine samples from 10 volunteers provided data on drug. results inferred that is absorbed rapidly relatively short half-life healthy individuals. obtained fitted 3P87 program pharmacokinetics. showed disposition conformed two-compartment open model Tmax 1.6 +/- 0.3 h, Cmax 109.8 38.7 ng/mL, T1/2 5.35 2.28 h AUC0-->infinity 322.1 69.6 ng/h/mI.