Proteins from disassembled microtubules characterized by oligospecific antisera.

摘要: The immunochemical properties of in vitro reassembled microtubules were investigated by immunoelectrophoretic techniques. tubulin dimer gave no measurable response, but the oligomer, tau-factor and an antigen about 135 000 daltons all precipitating antibodies. Those four proteins microtubules, DEAE-cellulose purified tubulin, after molecular sieve chromatography disassembled NaCl-dissociated microtubules. Reconstitution oligomer from was also performed. presence a unique antigenic structure on which not found dissociated components role this aggregate as nucleation center or intermediate assembly is discussed.