Identification of (−)-epicatechin as the direct substrate for polyphenol oxidase isolated from litchi pericarp

作者: Jian Sun , Yueming Jiang , Xiaoyi Wei , John Shi , Yanli You

DOI: 10.1016/J.FOODRES.2006.05.001

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摘要: Abstract Postharvest browning of litchi fruit results in a short life and reduced commercial value. The experiments were conducted to separate, purify identify the polyphenol oxidase (PPO) substrates that cause brown. PPO its were, respectively, extracted from pericarp tissues. for separated purified using polyamide column chromatography, silica gel chromatography preparative thin layer chromatography. substrate was further identified by 0.5% FeCl3 solution enzymatic reaction with PPO. On basis UV, 1H NMR, 13C ESI-MS data, direct tissues be (−)-epicatechin.

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