Development of innovative delivery systems to counteract vaginal infections

作者: Barbara Giordani

DOI: 10.6092/UNIBO/AMSDOTTORATO/9390

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摘要: The present thesis aimed to overcome some drawbacks associated with the currently available therapies for vaginal dysbiosis and use of conventional dosage forms. Formulations based on well-established drugs (chlorhexidine econazole nitrate) delivered in innovative systems formulations containing alternative active agents (probiotics cells polyphenols) have been taken into account. In paper 1 Bifidobacterium breve BC204 was isolated indagated its probiotic potential. B. subsequently protected by spray-drying encapsulation formulated inside orally time-dependent erodible lipid tablets, aim exert beneficial effects both at intestinal level. final forms provided high loading survival BC204, as well a delayed release mucoadhesive abilities. 2 chlorhexidine first complexed different polyanion polymers, then freeze-dried matrices either complex or along free drug polymers were prepared. Hyaluronate-based matrix showed flexible structure, best hydration properties, profile excellent antimicrobial properties. In 3 vaginally phosphatidylcholine vesicles biosurfactant, selected “green” surfactant, from strain. biosurfactant revealed peptide-like structure good surfactant emulsifying properties. Mixed presented optimal diameter range administration, efficiency mucoadhesion ability, sustained release. They also significantly improved ability eradicate Candida biofilm. In 4 liposomes two polyphenols (quercetin gallic acid) proposed antifungals problem azole resistance. They entrapment efficiencies better profiles compared only one polyphenol. Moreover, displayed strong anti-oxidant anti-inflammatory activities, resulted not cytotoxic inhibited proliferation.

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