Anti-EGFR-mediated radiosensitization as a result of augmented EGFR expression.

摘要: Abstract Purpose Elevated epidermal growth factor receptor (EGFR) expression has correlated with a poor prognosis after standard treatment of several malignancies. However, it is not clear whether the absolute level EGFR affects radiosensitizing properties anti-EGFR treatments. A better understanding this question would be helpful for design protocols that deliver these To explore question, cells (LS174T) did display inherent treatment–induced radiosensitization were selected studies could potentially enhance expression. Materials and methods Human colon carcinoma (LS174T), which show by treatments, employed studies. (Also, responsive to antiproliferative effects treatment.) Using transfection techniques (eukaryotic vector) as well an adenoviral construct expression, LS174T transduced in manner resulted enhanced EGFR. Subsequently, proliferation performed test (an monoclonal antibody: IMC-C225). Results Studies undertaken stably transfect The stable transfectants, LS174T.EGFR cells, treatment, contrast parent cells. Similar results demonstrated when infected AdEGFR. Additionally, (IMC-C225), whereas not. Conclusions Although prognostic significance many tumor models, response alone, or combinations radiation chemotherapy, depends upon factors are necessarily related reported herein, demonstrate increased they became