In vivo growth factor expansion of endogenous subependymal neural precursor cell populations in the adult mouse brain

摘要: The lateral ventricle subependyma in the adult mammalian forebrain contains both neural stem and progenitor cells. This study describes situ modulation of these subependymal precursor populations after intraventricular administration exogenous growth factors. In vivo infusion epidermal factor (EGF) into mouse for 6 consecutive days resulted a dramatic increase proliferation total number cells induced their migration away from walls adjacent parenchyma. Immediately EGF infusion, immunohistochemical characterization EGF-expanded cell population demonstrated that >95% were receptor- nestin-positive, whereas only 0.9% 0.2% labeled astrocytic neuronal markers, respectively. Seven weeks withdrawal, 25% to proliferate 6d still detectable; 28% had differentiated new astrocytes 3% neurons cortex, striatum, septum. Newly generated oligodendrocytes also observed. These results (1) confirm existence EGF-responsive (2) suggest acts directly as proliferation, survival, expand promote movement normal brain Thus, endogenous represents novel model studying development may provide insights will achieve replacement glia lost disease or trauma.