Multifunctional liposomes constituting microneedles induced robust systemic and mucosal immunoresponses against the loaded antigens via oral mucosal vaccination.

摘要: Abstract To develop effective, convenient and stable mucosal vaccines, mannose-PEG-cholesterol (MPC)/lipid A-liposomes (MLLs) entrapping model antigen bovine serum albumin (BSA) were prepared by the procedure of emulsification–lyophilization used to constitute microneedles, forming proMLL-filled microneedle arrays (proMMAs). The proMMAs rather hard enough pierce porcine skin and, upon rehydration, dissolved rapidly recovering MLLs without size entrapment change. given mice via oral (o.m.) route, than routine intradermal administration, elicited robust systemic immunoresponses against loaded antigens as evidenced high levels BSA-specific IgG in sera IgA salivary, intestinal vaginal secretions mice. Enhanced IgG2a IFN-γ treated revealed that induced a mixed Th1/Th2 immunoresponse. Moreover, significant increase CD8 + T cells confirmed strong cellular immunity had also been established immunization proMMAs. Thus, can be immunized o.m. route set up an effective multiple defense pathogen invasion may vaccine adjuvant-delivery system (VADS) applicable controlled temperature chain.