Hyphal Sheath and Iron-Binding Compound Formation in Liquid Cultures of Wood Decay Fungi Gloeophyllum trabeum and Postia placenta

摘要: Hyphal sheath (extracellular matrix) and iron-binding compound formation in liquid cultures of the brown rot fungi Gloeophyllum trabeum Postia placenta were examined as a function incubation temperatures, culture pH, concentrations iron, manganese, nitrogen medium. was observed microscopically quantified with Jandel video analysis (JAVA). The hyphal found on young rejuvenated hyphae, around tips along length but not necrotic hyphae. Production greater growing low metals (0-5μM iron or 0-100μM manganese) than under high metal concentrations. High decreased G. trabeum. Iron-binding formation, monitored by chrome azurol S (CAS) assay, did occur detectable quantities until third week incubation. production correlated metals, significantly influenced form source. levels manganese (1000-5000μM) (150-200μM) repressed both formation. Incubation temperature pH medium fungi. produced more iron-complexing agents at 3.5, 35°C other cultural conditions tested. yielded 5.5. 25°C Both over time, irrespective starting to final 2.4 3.0. Greatest decreases values seen iron. influence transition concentration experimental parameters ratio area, extracellular affinity compounds is significance because role these have been shown play biodegradation wood