Spontaneous development of IgM anti-cocaine antibodies in habitual cocaine users: effect on IgG antibody responses to a cocaine cholera toxin B conjugate vaccine.

摘要: Background and Objectives In cocaine vaccine studies, only a minority of subjects made strong antibody responses. To investigate this issue, IgG IgM responses to cholera toxin B (CTB—the carrier protein used enhance immune cocaine) were measured in sera from the 55 actively vaccinated Phase IIb randomized double-blind placebo-controlled trial (TA-CD 109). Methods Isotype specific ELISAs measure anti-cocaine anti-CTB serial samples collected prior at intervals after immunization. We assessed patients with pre-vaccination antibodies. Competitive inhibition ELISA was evaluate specificity. Results Conclusions Before immunization, 36/55 had detectable antibodies cocaine, 9 levels above 95% confidence limit 11 μg/ml. These nine significantly reduced peak 16 weeks, all below concentration (40 μg/ml) considered necessary discourage recreational use. The these same also reduced. Scientific Significance Subjects who develop an response course repeated exposure drug are less likely produce high conjugate vaccine. failure may be due induction type 2 T-cell independent response. Such individuals will require improved vaccines poor candidates for currently available (Am J Addict 2013;22:169-174)