Atomic Force Microscopy for DNA SNP Identification

摘要: The knowledge of the effects single-nucleotide polymorphisms (SNPs) in human genome greatly contributes to better comprehension relation between genetic factors and diseases. Sequence analysis genomic DNA different individuals reveals positions where variations that involve individual base substitutions can occur. Single-nucleotide are highly abundant have consequences at phenotypic level. Several attempts were made apply atomic force microscopy (AFM) detect map SNP sites strands. most promising approach is study mutations producing heteroduplex strands identifying mismatches by means a protein labels mismatches. MutS part well-known complex mismatch repair, which initiates process repairing when binds mismatched filament. position on filament be easily recorded AFM imaging.