Development and Validation of an Automated High-Throughput System for Zebrafish In Vivo Screenings
作者： Ainhoa Letamendia , Celia Quevedo , Izaskun Ibarbia , Juan M. Virto , Olaia Holgado
DOI: 10.1371/JOURNAL.PONE.0036690
关键词:
摘要: The zebrafish is a vertebrate model compatible with the paradigms of drug discovery. small size and transparency embryos make them amenable for automation necessary in high-throughput screenings. We have developed an automated platform vivo chemical screenings on that includes methods embryo dispensation, compound delivery, incubation, imaging analysis results. At present, two different assays to detect cardiotoxic compounds angiogenesis inhibitors can be automatically run platform, showing versatility system. A validation these known positive negative compounds, as well screening detection unknown anti-angiogenic been successfully carried out system developed. present totally allows organism.
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paperity.org参考文章(58)
Ravindra Peravali, Jochen Gehrig, Stefan Giselbrecht, Dominic S. Lütjohann, Yavor Hadzhiev, Ferenc Müller, Urban Liebel, Automated feature detection and imaging for high-resolution screening of zebrafish embryos BioTechniques. ,vol. 50, pp. 319- 324 ,(2011) , 10.2144/000113669
GreggR Ward, AbdelA Abdel-Rahman, Orchiectomy or androgen receptor blockade attenuates baroreflex-mediated bradycardia in conscious rats BMC Pharmacology. ,vol. 6, pp. 2- 2 ,(2006) , 10.1186/1471-2210-6-2
Renhai Cao, Ebba Bråkenhielm, Xuri Li, Kristian Pietras, Johan Widenfalk, Arne Östman, Ulf Eriksson, Yihai Cao, Angiogenesis stimulated by PDGF-CC, a novel member in the PDGF family, involves activation of PDGFR-αα and -αβ receptors The FASEB Journal. ,vol. 16, pp. 1575- 1583 ,(2002) , 10.1096/FJ.02-0319COM
Carolyn A. Staton, Malcolm W. R. Reed, Nicola J. Brown, A critical analysis of current in vitro and in vivo angiogenesis assays International Journal of Experimental Pathology. ,vol. 90, pp. 195- 221 ,(2009) , 10.1111/J.1365-2613.2008.00633.X
Douglas Hanahan, Signaling Vascular Morphogenesis and Maintenance Science. ,vol. 277, pp. 48- 50 ,(1997) , 10.1126/SCIENCE.277.5322.48
Peter Carmeliet, Rakesh K. Jain, Angiogenesis in cancer and other diseases Nature. ,vol. 407, pp. 249- 257 ,(2000) , 10.1038/35025220
Alexander Kamb, What's wrong with our cancer models? Nature Reviews Drug Discovery. ,vol. 4, pp. 161- 165 ,(2005) , 10.1038/NRD1635
Napoleone Ferrara, Kari Alitalo, None, Clinical applications of angiogenic growth factors and their inhibitors Nature Medicine. ,vol. 5, pp. 1359- 1364 ,(1999) , 10.1038/70928
Jean Giacomotto, Laurent Ségalat, High-throughput screening and small animal models, where are we? British Journal of Pharmacology. ,vol. 160, pp. 204- 216 ,(2010) , 10.1111/J.1476-5381.2010.00725.X
Dong Liang, Jenny R Chang, Alvin J Chin, Alastair Smith, Christina Kelly, Eric S Weinberg, Ruowen Ge, None, The role of vascular endothelial growth factor (VEGF) in vasculogenesis, angiogenesis, and hematopoiesis in zebrafish development Mechanisms of Development. ,vol. 108, pp. 29- 43 ,(2001) , 10.1016/S0925-4773(01)00468-3