Comparison of two hybridization assays for the rapid detection of PCR amplified HSV genome sequences from cerebrospinal fluid

摘要: Abstract Rapid diagnosis of herpes simplex encephalitis (HSE) can only be achieved by the polymerase chain reaction (PCR). In order to carry out PCR under routine conditions, it is great importance establish an easy DNA extraction protocol and especially a rapid sensitive detection method. present study, two different solid phase hybridization assays (Gen-Eti-K-DNA Enzyme Immunoassay (DEIA), Sorin Biomedica, Italy Enzymun-Test detection, Boehringer Mannheim, Germany) were compared for amplified HSV genome region, using standard primers, from cerebrospinal fluid (CSF) samples. 122 CSF samples obtained patients suffering hospitalized at University Clinics Frankfurt Graz during period January 1992 July 1993 tested. To ascertain sensitivity assays, dilution series plasmid, encoding region gene, investigated. The limit DEIA assay was one copy plasmid/μl, lowest amount which could detected Enzymun as well Southern blot 10 copies/μl. 15 with HSE found positive three assays. Concordant results also non-HSE patients. this study show that new systems guarantee fast high-sensitive DNA. in carried routinely combined use simple procedure.