An in vitro study of the susceptibility of mobile and cystic forms of Borrelia burgdorferi to tinidazole.

摘要: The susceptibility of mobile and cystic forms Borrelia burgdorferi to tinidazole (TZ) was examined. minimal bactericidal concentration (MBC) TZ against the spirochetes >128 μg/ml at 37°C in micro-oxic atmosphere when incubated for 14 days. significantly reduced conversion during incubation. MBC older (10- months-old) cysts a >0.5 μg/ml, but >0.125 young (1-day-old) cysts. Acridine orange staining, dark-field microscopy transmission electron revealed that, ≥ MBC, contents were partly degraded, core structures did not develop inside cysts, amount RNA these decreased significantly. When exposed TZ, both spirochetal dissolved, production blebs reduced. These observations may be valuable treatment resistant infections caused by B. burgdorferi, suggest that combination macrolide antibiotic could eradicate burgdorferi. [Int Microbiol 2004; 7(2):139–142]