Clonal Expansion of Double-Positive Intraepithelial Lymphocytes by MHC Class I-Related Chain A Expressed in Mouse Small Intestinal Epithelium
作者： Eun Jeong Park , Ichiro Takahashi , Junko Ikeda , Kazuko Kawahara , Tetsuji Okamoto
DOI: 10.4049/JIMMUNOL.171.8.4131
关键词:
摘要: Expression of a distant homologue MHC class I molecule, I-related chain A (MICA), has been found to be stress inducible and limited the intestinal epithelium. This nonclassical molecule is associated with various carcinomas in humans. To understand biological consequences MICA expression gut, we generated transgenic (Tg) mice (T3(b)-MICA Tg) under control T3(b) promoter. The T3(b)-MICA Tg expressed selectively intestine had an increased number TCRalphabeta CD4CD8alphaalpha, double-positive (DP) intraepithelial lymphocytes (IELs) small bowel. These MICA-expanded DP IELs exhibited bias Vbeta8.2 overlapped motifs complementarity-determining region 3 among mice. Hence, overexpression resulted clonal expansion IELs. Studies model inflammatory bowel disease showed that was able attenuate acute colitis induced by dextran sodium sulfate administration. Therefore, this unique vivo will enable investigation possible influences stress-inducible on gut immune surveillance.
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