Chemical Mechanisms for Mobilizing DNA

摘要: Different types of elements, and their associated recombinases, continue to be discovered characterized. Studies two major classes these recombinases have advanced the point that detailed molecular mechanisms can discussed. This chapter focuses on protein-catalyzed recombination by provide examples how chemistry DNA mobility is catalyzed controlled. Central both rearrangements are polynucleotidyl transfer steps. The likely catalytic functions several active-site residues. key understanding used transposases knowledge different segments positioned repositioned within active sites as progresses. characteristic related important biological features distinguish proteins from traditional enzymes. First, physiological demand reaction rate for phosphoryl in usually very low, with many reactions occurring less than once every cell cycle. Second, physiology also frequently demands essentially all substrate molecules converted product, even when "substrate" "product" nearly isoenergetic. structural organization complexes intimately control. Perhaps one most satisfying aspects continued site-specific transposition that, details protein-DNA complex structures strategies elucidated, they insight into basis regulation.