The influence of leptin on the activity of lung lymphocytes under simulated microgravity

摘要: Exposure to microgravity has been implicated in the compromised immune function space travellers, resulting opportunistic infections, poor wound healing, and cancer. Since recent studies have suggested that leptin was capable of modulating responses, purpose this study examine effects on activation proliferation rat lung lymphocytes then leptin-mediated signal transduction mechanisms lymphocyte these same conditions. In control conditions (T-flasks cultured cells) not able by itself increase proliferation, or induce significant either IL-2 production expression markers, such as CD25 CD71, while it markedly enhanced positive induced parameters concanavalin A (ConA). Using clinostatic rotating wall vessel (RWV) bioreactors simulate a environment, we found ConA responsiveness inhibited. Moreover, under conditions, reverse impaired functions. Accordingly with above cited inhibitory exerted simulated evidence also obtained defects intracellular incubation RWV bioreactors, namely concerning decreased ConA-mediated PKC activity, reduced NF-κB, c-fos, ERK1/2. Again, appeared be unable restoring physiologic parameters, different from what could observed after complementation signalling phorbol myristate acetate, which instead demonstrated overcome inhibition activating functions, presence