Advanced Sperm Processing/Selection Techniques

作者: Ashok Agarwal , Manesh Kumar Panner Selvam

DOI: 10.1007/978-3-319-71815-6_28

关键词:

摘要: DNA damage is considered to be one of the main factors linked with male infertility and successful human reproduction partly relies on integrity sperm DNA. Assisted reproductive technique (ART) a treatment choice for female mainly processing selection techniques select superior-quality spermatozoa. Even after development different spermatozoa, success rates remain below expectation. Sperm higher fragmentation are associated poor fertilization cleavage leading poor-quality embryo early pregnancy loss. Conventional cannot spermatozoa based their chromatin structure. Certain novel advanced such as electrophoresis, zeta potential, molecular binding techniques, morphological evaluation under high magnification, microfluidics able intact Therefore, this chapter deals developed use in ART. Additionally we have discussed confocal light absorption scattering spectroscopic microscopy, interferometric phase Raman spectrometry, synthetic oligopeptide (DWI) staining, which might useful future.

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