Ribotyping of Indian Isolates of Pasteurella multocida Based on 16S and 23S rRNA Genes

作者: M. K. Saxena , A. A. Kumar , P. Chaudhari , S. B. Shivachandra , V. P. Singh

DOI: 10.1007/S11259-005-1864-5

关键词:

摘要: The applicability of ribotyping based on 16S and 23S rRNA was evaluated for molecular epidemiological studies. Forty-eight isolates Pasteurella multocida isolated from different hosts geographical locations one reference isolate were ribotyped. Only four ribotypes found. All the including wild carnivores had same ribotype, though they serotypes. a tiger band in addition to bands present major ribotype. lions represented two ribotypes; these ribotypes, (r2) an additional 3.6 kbp, which absent all other ribotypes. second ribotype (r4) lion missing (6 kbp) that These further typed using ERIC-PCR REP-PCR. With REP-PCR, higher D values 0.83 0.89 obtained. current study revealed is not very efficient typing tool use epidemiology differentiation isolates.

参考文章(16)
Kakambi V Nagaraja, C. J. Kim, DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys. American Journal of Veterinary Research. ,vol. 51, pp. 207- 210 ,(1990)
MM Chawak, KC Verma, JM Kataria, AA Kumar, None, Characterization of indigenous isolates of avian Pasteurella multocida. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases. ,vol. 21, pp. 111- 114 ,(2000)
Kate Wilson, Preparation of Genomic DNA from Bacteria Current protocols in molecular biology. ,vol. 56, ,(2001) , 10.1002/0471142727.MB0204S56
M A Wilson, R B Rimler, L J Hoffman, Comparison of DNA fingerprints and somatic serotypes of serogroup B and E Pasteurella multocida isolates. Journal of Clinical Microbiology. ,vol. 30, pp. 1518- 1524 ,(1992) , 10.1128/JCM.30.6.1518-1524.1992
Meredith L. Hunt, Carmel G. Ruffolo, Kumar Rajakumar, Ben Adler, Physical and Genetic Map of the Pasteurella multocida A:1 Chromosome Journal of Bacteriology. ,vol. 180, pp. 6054- 6058 ,(1998) , 10.1128/JB.180.22.6054-6058.1998
PJ Blackall, JK Miflin, JK Miflin, Identification and typing of Pasteurella multocida: a review. Avian Pathology. ,vol. 29, pp. 271- 287 ,(2000) , 10.1080/03079450050118395
B. Regnault, F. Grimont, P.A.D. Grimont, Universal ribotyping method using a chemically labelled oligonucleotide probe mixture. Research in Microbiology. ,vol. 148, pp. 649- 659 ,(1997) , 10.1016/S0923-2508(99)80064-3
P. J. Blackall, N. Fegan, G. T. I. Chew, D. J. Hampson, Population structure and diversity of avian isolates of Pasteurella multocida from Australia Microbiology. ,vol. 144, pp. 279- 289 ,(1998) , 10.1099/00221287-144-2-279