A study of mechanisms of carcinogenesis by gene transfer of oncogenes into mammalian cells (MTR 07215)

摘要: Recent work has shown that individual oncogenes can be involved in several steps of the multistage process carcinogenesis. Evidence comes from studies on expression cloned transfected into early passage mammalian cells and immortalized non-tumorigenic cell lines. Transformation epithelial vitro with cellular viral is special interest since most human tumors are origin. An important aspect transformation by induction transforming growth factors (TGFs). The role differentiation been examined introducing myc mutant T24 Ha-ras1 genes mouse erythroleukemic which were then induced to differentiate. In clones was inhibited or ras genes. Studies reported using linked transcriptional control elements regulated vitro, such as metallothionein (hMT-IIA) promoter region, cadmium dexamethasone. Phenotypic properties transfectants including morphological transformation, anchorage dependence TGF release dependent regulators hMT-IIA region.