Detection of polyol accumulation in a new ovarian carcinoma cell line, CABA I: a(1)H NMR study.

摘要: Ovarian carcinomas represent a major form of gynaecological malignancies, whose treatment consists mainly surgery and chemotherapy. Besides the difficulty prognosis, therapy ovarian has reached scarce improvement, as consequence lack efficacy development drug-resistance. The need different biochemical functional parameters grown, in order to obtain larger view on processes biological clinical significance. In this paper we report novel metabolic features detected series human ovary carcinoma lines, by 1H NMR spectroscopy intact cells their extracts. Most importantly, new adenocarcinoma line CABA I, showed strong signals spectral region between 3.5 4.0 p.p.m., assigned for first time polyol sorbitol (39±11 nmol/106 cells). 13C analyses these incubated with [1-13C]-D-glucose demonstrated labelled-sorbitol formation. other cell lines (OVCAR-3, IGROV 1, SK-OV-3 OVCA432), showed, same region, intense resonances from metabolites: glutathione (up 30 nmol/106 cells) myo-inositol 50 nmol/106 cells). Biochemical functions are suggested compounds cells, especially relation possible role detoxification mechanisms during tumour progression.